This web page was produced as an assignment for Genetics 564, an undergraduate capstone course at UW-Madison.
What are post-translational modifications?
Post-translational modifications are chemical modifications that affect the functions of a protein by altering the activity regulation, localization, and interactions with other molecules. [1] Post-translational modifications are mediated by enzymes including: kinases, phosphatases, other proteins, lipids, sugars and proteases. Modifications can occur at any stage in the protein life cycle and can shed light on the underlying etiological processes.
Types of post-translational modifications
Post-translational modifications alter the structure of proteins, altering the activity, stability, localization, and interaction with other molecules. Below are some of these types of post-translational modifications.
Post-Translational Modification of CRY1
Bioinformatics server NetPhos was used to predict serine, threonine and tyrosine phosphorylation sites in human, mouse, and zebrafish protein sequences.
Conclusion
Phosphorylation sites of CRY1 protein between species are varied, exhibiting the possibility for different regulation patterns and functions for the protein. There is some conservation in the 100 to 300 sequence positions, where the photolyase domain resides, and in the 400 sequence position across the three organisms, specifically, for serine phosphorylation, displaying an area of interest for studies of the role of serine phosphorylation in the latter part of the protein.
References.
1. Overview of Post-Translational Modification. (n.d.). Retrieved from https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-post-translational-modification.html
2. Post-Translational Modification (PTM) Antibodies. (n.d.). Retrieved from https://rockland-inc.com/post-translational-modification-antibodies.aspx
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1. Overview of Post-Translational Modification. (n.d.). Retrieved from https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-post-translational-modification.html
2. Post-Translational Modification (PTM) Antibodies. (n.d.). Retrieved from https://rockland-inc.com/post-translational-modification-antibodies.aspx
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Sara Acosta Villarreal Genetics and Genomics, UW-Madison [email protected] Last updated: May 10, 2019 |
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